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코로나바이러스 SARS-CoV-2의 신속한 진단을 위한 RT-LAMP


SMART
 

코로나바이러스 SARS-CoV-2의 신속한 진단을 위한 RT-LAMP

Wei E. Huang, Boon Lim, Chia-Chen Hsu, Dan Xiong, Wei Wu, Yejiong Yu, Huidong Jia, 외 | 아진

출간일
2020-07-12
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콘텐츠 소개
목차
한줄서평

콘텐츠 소개

The pandemic coronavirus SARS-CoV-2 in the world has caused a large infected
population suffering from COVID-19. To curb the spreading of the virus, WHO
urgently demanded an extension of screening and testing; thus, a rapid and simple
diagnostic method is needed. We applied a reverse transcription-loop-mediated
isothermal amplification (RT-LAMP) to achieve the detection of SARS-CoV-2 in 30
min. We designed four sets of LAMP primers (6 primers in each set), targeting the
viral RNA of SARS-CoV-2 in the regions of orf1ab, S gene and N gene. A
colorimetric change was used to report the results, which enables the outcome of
viral RNA amplification to be read by the naked eye without the need of expensive
or dedicated instrument. The sensitivity can be 80 copies of viral RNA per ml in a
sample. We validated the RT-LAMP method in a hospital in China, employing 16
clinic samples with 8 positives and 8 negatives. The testing results are consistent
with the conventional RT-qPCR. In addition, we also show that one-step process
without RNA extraction is feasible to achieve RNA amplification directly from a
sample. This rapid, simple and sensitive RT-LAMP method paves a way for a large
screening at public domain and hospitals, particularly regional hospitals and
medical centres in rural areas.

목차

제 1편 코로나바이러스 정의
1. 코로나바이러스감염증-19(Covid-19) 정보 7
2. 코로나바이러스 분류 및 특성 9
3. 코로나바이러스 전자현미경 형태 11
4. 코로나바이러스 구조 (Covid-19 Organization) 13
5. 코로나19: 환경에 지속적인 영향을 미칠까? 19
6. 치료법(Therapeutical Method) 22

제 2편 연구논문
RT-LAMP for rapid diagnosis of coronavirus SARS-CoV-2

1. Introduction 23
2. Results 24
3. One-step process of nucleic acid detection using
RT-LAMP 28
4. Discussion 28
5. Experimental procedures 31
6. DNA and RNA purification 32
7. RT-LAMP for clinical RNA samples 33
8. References 33

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